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KMID : 1188320130070010030
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Gut and Liver
2013 Volume.7 No. 1 p.30 ~ p.34
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Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test
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Kim Ho-Dong
Kim Do-Hyun
Park Hye-uk
Kim Woo-Jong
Ahn Yong-Soo
Lee Young-Jik
Park Sun-Mi
Seo Eun-Seon
Park Chul
Kim Yang-Ho
Kim Hyung-Rag
Joo Young-Eun
Jung Young-Do
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Abstract
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Background/Aims:The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.
Methods:Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.
Results:Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.
Conclusions:The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.
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KEYWORD
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Helicobacter pylori, Gastric aspirate, Monoclonal antibody-based test
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